The method of claim 18, further comprising prior to lysing the microalgae, pretreating the microalgae to reduce their bursting strength.Ģ0. A method of lysing algae cells, comprising flowing the microalgae in the aqueous medium through the lysing structure of claim 1.ġ9. The method of claim 16, wherein the microalgae are pretreated with either a raised temperature or lowering pH to reduce their bursting strength.ġ8. The method of claim 14, further comprising prior to lysing the microalgae, pretreating the microalgae to reduce their bursting strength.ġ7. The method of claim 14, wherein the product is a biofuel that is prepared from a hydrophobic fraction of the lysate.ġ6. lysing the microalgae to produce a lysate by flowing the microalgae in the aqueous medium through the lysing structure of claim 1 and c. growing microalgae in an aqueous medium in a photobioreactor b. A method of preparing a product from an algae lysate comprising: a. The system of claim 1, in which the lysing structure at the passive flow rate produces turbulent flow in the lysing structure and walls of the flow path comprise a surface roughness extending into the flow path beyond a turbulent flow viscous sublayer for the medium at the passive flow rate.ġ4. The system of claim 1, in which the flow-altering structure is a bend in the flow path.ġ3. The system of claim 1, in which the flow-altering structure is a venturi.ġ2. The system of claim 1, in which the flow-altering structure is a restriction in a diameter of the flow path.ġ1. The system of claim 1, in which the flow-altering structure is a protuberance within the flow path.ġ0. The system of claim 1, further comprising one or more additional lysing structures in the fluid conduit.ĩ. The system of claim 6, in which the flow rate is generated by from 8 J to 48,000 J of energy per Liter of algae culture in algae culture medium.Ĩ. The system of claim 1, in which the flow rate is generated by 48,000 J or less of energy per Liter of algae culture in algae culture medium.ħ. The system of claim 1, in which the flow rate is generated by a gravitational head of water of between 8 and 40 meters.Ħ.
The system of claim 1, in which the flow rate is produced by a pressure of 60 psi or less.ĥ. The system of claim 1, in which the flow rate is less than or equal to a gravity flow rate.Ĥ. The system of claim 1, comprising algae in algae culture medium within the reservoir at a level above the fluid outlet of the reservoir.ģ. a fluid conduit attached to the fluid outlet and comprising one or more lysing structures comprising a fluid flow path and a flow-altering structure that modifies the flow path to generate sufficient force to lyse an algae cell in the algae culture in at least a portion of the flow path at a flow rate generated by either a gravitational head of water of 40 meters or less, 60 psi or less, 30 W or less, or 48,000 Joules (J) or less of energy per Liter of algae culture in algae culture medium.Ģ.
a reservoir having a fluid outlet adapted for removal of fluid from the reservoir b. system for producing an algae lysate from an algae culture in an algae culture medium, comprising: a. WISE/SOST NUCLEIC ACID SEQUENCES AND AMINO ACID SEQUENCESġ. Method for reducing the immunogenicity of heterologous proteins by elimination of t-cell epitopes Renilla reniformis green fluorescent protein and mutants thereofĭual antigen specific T cells with trafficking ability Nucleic acid sequences encoding novel point mutations on mglur2 and mglur3ĬOMPOSITIONS AND METHODS COMPRISING SERINE PROTEASE VARIANTS (AS AMENDED) GENETICALLY MODIFIED HOST CELLS FOR INCREASED P450 ACTIVITY LEVELS AND METHODS OF USE THEREOF Methods and Compositions for Correlating Genetic Markers with Sudden Cardiac Death RiskĭEVICES, COMPOSITIONS, AND METHODS FOR MEASURING MOLECULES AND FORCES Identification of polynucleotides for predicting activity of compounds that interact with and/or modulate protein tyrosine kinases and/or protein tyrosine kinase pathways in lung cancer cells
Secreted and transmembrane polypeptides and nucleic acids encoding the same